SART1 Antibody

SKU: orb1255762

Description

SART1 Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1).
• Target: SART1
• Molecular Weight: Observed: 120kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

SART1, squamous cell carcinoma antigen recognised by T cells, ARA1, Ara1, HOMS1, MGC2038, SART1259, Snu66, IgE autoantigen, SART1(259) protein, SART1(800) protein, U4/U6.U5 tri-snRNP-associated 110 kDa protein, squamous cell carcinoma antigen recognized by T cells 1, SNRNP110

Western blot analysis of extracts of various cell lines, using SART1 antibody (orb1255762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunoprecipitation analysis of 150 ug extracts of Jurkat cells using 3 ug SART1 antibody (orb1255762). Western blot was performed from the immunoprecipitate using SART1 antibody (orb1255762) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

SART1

UniProt

O43290