SART1 Antibody

• Catalog Number: orb1255762
• Category: Antibodies
• Description: SART1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SART1 (NP_005137.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 120kDa
• Target: SART1
• UniProt ID: O43290
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SART1, squamous cell carcinoma antigen recognised
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SART1 antibody (orb1255762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunoprecipitation analysis of 150 ug extracts of Jurkat cells using 3 ug SART1 antibody (orb1255762). Western blot was performed from the immunoprecipitate using SART1 antibody (orb1255762) at a dilution of 1:1000.