SARS-CoV Matrix Antibody

• Catalog Number: orb1239027
• Category: Antibodies
• Description: SARS-CoV Matrix Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2/SARS-CoV Matrix antibody (orb1239027) was raised against a peptide corresponding to 15 amino acids near the carboxy-terminus of SARS-CoV Matrix protein. The immunogen is located within the last 50 amino acids of SARS-CoV Matrix.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL.Antibody validated: SARS-CoV-2/SARS-CoV Matrix antibody can be used for the detection of SARS-CoV-2/SARS-CoV Matrix protein in ELISA. It will detect 5 ng of free peptide at 1 μg/mL.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: M
• UniProt ID: O62114
• NCBI: QHO62114.1
• Storage: SARS-CoV-2/SARS-CoV Matrix antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2/SARS-CoV Matrix Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Ma
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with SARS-CoV-2 Matrix Recombinant Protein. Loading: SARS-CoV-2 Matrix recombinant protein. Antibodies: SARS-CoV-2/SARS-CoV Matrix (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 10 ng Matrix protein, Lane 2: 25 ng Matrix protein and Lane 3: 50 ng Matrix protein.

ELISA Test. Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, orb1239027. A direct ELISA was performed using Matrix recombinant protein as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Detection range is from 8 ng/mL to 2000 ng/mL.

ELISA Test. Antibodies: SARS-CoV-2/SARS-CoV Matrix Antibody, orb1239027 (1 µg/mL). A direct ELISA was performed using immunogen (orb1239027) as coating antigen and the anti-SARS-CoV-2/SARS-CoV Matrix antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.