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Catalog Number | orb1954176 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 Spike S1 Protein (D614G, His) is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.41 kDa and the accession number is A0A6G7K2L4. |
Tag | C-His |
Purity | 98.00% |
UniProt ID | A0A6G7K2L4 |
MW | 76.41 kDa (predicted) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | SARS-CoV-2 |
Biological Activity | SARS-CoV-2 Spike S1 Protein (D614G, His) is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.41 kDa and the accession number is A0A6G7K2L4. |
Expression Region | A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein (YP_009724390.1) (Val16-Arg685(D614G)) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Val16 |
Storage | -20°C |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
> 90% as determined by SDS-PAGE | |
120-140 kDa |
> 90% as determined by SDS-PAGE | |
90 kDa |
> 95% as determined by SDS-PAGE | |
110-130 kDa |
410.37 kDa; Protein contains amino acids 14-1211 and following mutations: T19R, G142D, E156G, del_F157-R158 L452R, T478K, D614G, P681R. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). | |
CHO-based cell line (expressed by QMCF Technology) |
> 90% as determined by SDS-PAGE | |
110-130 kDa |