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Catalog Number | orb1953722 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 Spike RBD Protein (T478K, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.7 kDa and the accession number is A0A6G7K2L4. |
Tag | C-His |
Purity | 98.00% |
UniProt ID | A0A6G7K2L4 |
MW | 26.7 kDa (predicted); 34.9 kDa (reducing condition, due to glycosylation) |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expression System | HEK293 Cells |
Biological Origin | SARS-CoV-2 |
Biological Activity | SARS-CoV-2 Spike RBD Protein (T478K, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.7 kDa and the accession number is A0A6G7K2L4. |
Expression Region | A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation T478K) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutation were identified in the SARS-CoV-2 variant (known as variant B.1.617.2) which emerged in the India. Predicted N terminal: Ser |
Storage | -20°C |
Note | For research use only |
> 90% as determined by SDS-PAGE | |
36 kDa |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 26.0 kDa after removal of the signal peptide. The apparent molecular mass of RBD(L452R,T478K)-His is approximately 25-35 kDa due to glycosylation. | |
Mammalian |
> 90% as determined by SDS-PAGE | |
36 kDa |
> 90% as determined by SDS-PAGE | |
36 kDa |
> 90% as determined by SDS-PAGE | |
36 kDa |