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| Catalog Number | orb1231115 |
|---|---|
| Category | Proteins |
| Description | SARS-CoV-2 (COVID-19) ORF8 Recombinant Protein |
| Target | 8 |
| Tag | N-Term MBP Uncleaved |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | 50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35 |
| Purity | Amylose Resin (0.95) |
| Protein Sequence | Native Sequence:MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSMKFLVFLGIITTVAAFHQECSLQSCTQHQPYVVDDPCPIHFYSKWYIRVGARKSAPLIELCVDEAGSKSPIQYIDIGNYTVSCLPFTINCQEPKLGSLVVRCSFYEDFLEYHDVRVVLDFIAmino acids M1 – I121 (end).Residue M404 of the fusion protein is equivalent to M1 of the native enzyme.The MBP tag is located at residues 1 – 367.Protease Cleavage:PreScission (LEVLFQGP) residues 393 – 400 |
| UniProt ID | P0DTC8 |
| MW | Mono-Isotopic Mass: 58, 033.24 daltons Average Mass: 58, 069.92 daltons |
| Source | bacteria |
| Storage | Store at -70 °C. Avoid repeated freeze-thaw cycles. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Alternative names | SARS-CoV2-ORF8, SARS-CoV-2 ORF8, ORF8 SARS-CoV-2 |
| Research Area | Infectious Disease & Virology |
| Note | For research use only |
| NCBI | QHD43422.1 |

GSH-Agarose (0.95) | |
Mono-Isotopic Mass: 40, 628.51 daltons Average Mass: 40, 655.17 daltons | |
bacteria |
ELISA, WB | |
>80% as estimated by SDS-PAGE gel | |
13.4 kD | |
E. coli |
≥ 90% as determined by SDS-PAGE | |
Recombinant SARS-CoV-2 ORF8 protein was expressed from E.coli expression system and the target gene encoding Phe16-IIe121 is expressed with N-His Tag |
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