SARS-CoV-2 (COVID-19) Nucleocapsid protein

• Catalog Number: orb638880
• Category: Proteins
• Description: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
• Reactivity: Virus
• Tag: N-6×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 49.4 kDa
• Target: Nucleocapsid protein
• Protein Sequence: QHO62115.1
• Source: SARS-CoV-2 Nucleocapsid protein, His Tag (orb638880) is expressed from E. coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1).
• Expression System: E. coli
• Expression Region: Met 1 - Ala 419
• Endotoxins: 1.0 EU per μg
• NCBI: QHO62115.1
• Storage: -20℃
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, Arginine, pH7.5
• Alternative names: Nucleocapsid protein,NP,Protein N
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 49.4 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.3 ng/mL (QC tested).

SARS-CoV-2 Nucleocapsid protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.