SARS-CoV-2 (COVID-19) Nucleocapsid Protein, His Tag

• Catalog Number: orb638880
• Category: Proteins
• Description: SARS-CoV-2 Nucleocapsid protein, His Tag is expressed from E. coli cells. It contains AA Met 1 - Ala 419 (Accession # QHO62115.1).
• Target: Nucleocapsid protein
• Conjugation: Unconjugated
• Tag: N-6×His
• Reactivity: Virus
• Form/Appearance: Powder
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, Arginine, pH 7.5
• Purity: 90%
• Protein Sequence: QHO62115.1
• MW: 49.4 kDa
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 49.4 kDa. The protein migrates as 47-50 kDa under reducing (R) condition (SDS-PAGE).
• Expression System: E. coli
• Endotoxins: 1.0 EU per μg
• Biological Origin: SARS-CoV-2
• Expression Region: Met 1 - Ala 419
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only
• NCBI: QHO62115.1

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.3 ng/mL (QC tested).

SARS-CoV-2 Nucleocapsid protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.