SARS-CoV-2 (COVID-19) Nucleocapsid Antibody

• Catalog Number: orb1239996
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (orb1239996) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Nucleocapsid protein. The immunogen is located within 350-400 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-2 μg/mLSARS-CoV-2 (COVID-19) Nucleocapsid antibody can be used for the detection of SARS-CoV-2 (COVID-19) Nucleocapsid protein in ELISA. It will detect 4 ng of free peptide at 1 μg/mL. Antibody can also be used for as capture antibody in Sandwich Elisa at 10 μg/mL. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: N
• NCBI: QHD43423
• Storage: SARS-CoV-2°COVID-19) Nucleocapsid antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody: Sever
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein, orb1231585. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, orb1239996, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.

Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein. Loading: 50 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, orb1239996, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL.

ELISA Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid antibody, orb1239996 (1 µg/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.

ELISA Validation of SARS-CoV-2 Nucleocapsid Antibody Pair. A sandwich ELISA was performed using the anti-SARS-COV-2 Nucleocapsid antibodies orb1239996 (10 µg/mL) as the capture antibody. Biotin-labled anti-SARS-COV-2 Nucleocapsid antibodies orb1239067 (1 µg/mL) and streptavidin-HRP (0.1 µg/mL) were used for detection. Detection range is from 3 ng to 200 ng (SARS-CoV-2 Nucleocapsid Protein, orb1231585).

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1273021 and orb1239996. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1273021, 5 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1239996, 1 ug/ml) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng.