SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein

• Catalog Number: orb1231585
• Category: Proteins
• Description: SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein
• Tested applications: ELISA, WB
• Tag: His tag at C-terminus
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Purity: < 95%
• MW: 50 kD
• Protein Sequence: Met1-Ala419
• Source: E. coli
• Storage: The protein is stable in a liquid state at -70°C for 12 months. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS
• Alternative names: SARS-CoV-2 N protein, N phosphoprotein, Nucleocaps
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein, Loading: 1 μg SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein, orb1231585. Observed: 50kD

ELISA Validation of SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein. Coating Antigen: SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein (orb1231589) from 0.3 ng to 1000 ng. Capture Antibody: SARS-CoV-2 (COVID-19) Nucleocapsid antibody, orb1239972 (1 μg/mL). Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution.

Western Blot Validation with SARS-CoV-2 (COVID-19) Nucleocapsid Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Nucleocapsid recombinant protein, orb1231585. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid, orb1239996, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mLand Lane 2: 2 μg/mL.