SARS-CoV-2 (COVID-19) NSP1 Recombinant Protein

Catalog Number: orb1231052

Catalog Number	orb1231052
Category	Proteins
Description	SARS-CoV-2 (COVID-19) NSP1 Recombinant Protein
Tag	N-Term GST Uncleaved
Concentration	batch dependent
Form/Appearance	Liquid
Purity	GSH-Agarose (0.5)
MW	Mono-Isotopic Mass: 46, 569.73 daltons Average Mass: 46, 599.47 daltons
Target	ORF1ab
UniProt ID	P0DTC1
Protein Sequence	Native Sequence:MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGGAmino acids M1 – G180 (end). Residue M232 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.Protease Cleavage:PreScission (LEVLFQGP) residues 221 - 228
Source	bacteria
NCBI	QHD43415.1
Storage	Store at -70 °C. Avoid repeated freeze-thaw cycles.
Buffer/Preservatives	50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35
Alternative names	SARS-CoV2-NSP1, SARS-CoV-2 NSP1, NSP1 SARS-CoV-2 Read more...
Note	For research use only
Expiration Date	6 months from date of receipt.

Purity:	> 85% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining.
MW:	21.94kDa
Source:	E. coli

Purity:	Amylose Resin (0.6)
MW:	Mono-Isotopic Mass: 63, 974.47 daltons Average Mass: 64, 014.22 daltons
Source:	bacteria

Purity:	Greater than 85% as determined by reducing SDS-PAGE.
Source:	E. coli

Purity:	≥ 90% as determined by SDS-PAGE
Source:	Recombinant SARS-CoV-2 NSP1 was expressed from E.coli expression system and the target gene encoding Met1-Gly180 is expressed with N-His Tag