SALL1 antibody

• Catalog Number: orb20344
• Category: Antibodies
• Description: Goat polyclonal antibody to SALL1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 1 μg/ml
• Conjugation: Unconjugated
• MW: 140; 130
• Target: SALL1 (aa29-43)
• Entrez: 6299
• Protein Sequence: EKGQPSRPTKSKDAH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SALL1 antibody, anti sal-like 1 (Drosophila)
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Blot: Preliminary experiments gave an approx 100kDa band in Human Brain (Amygdala and Substantia nigra) and Mouse Kidney lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 140kDa according to Human NP_002959.2 and Mouse NP_067365.2, and 130kDa according to Human NP_001121364.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb20344 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb20344 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).