SAHH Rabbit Polyclonal Antibody

• Catalog Number: orb6916
• Category: Antibodies
• Description: SAHH Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SAHH (25-125/432aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 48 kDa
• Target: AHCY
• UniProt ID: P23526
• RRID: AB_10923564
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Adenosylhomocysteinase; AdoHcyase; ahcY; S adenosy
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-SAHH antibody (orb6916), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (SAHH) Polyclonal Antibody, Unconjugated (orb6916) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

SAHH was immunoprecipitated from mouse kidney tissue with orb6916 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution. Lane 1: mouse kidney tissue lysate 10 µg (Input). Lane 2: orb6916 IP in mouse kidney tissue lysate. Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control). Secondary, All lanes: Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000.

Sample: Kidney (Mouse) Lysate at 40 ug Skin (Mouse) Lysate at 40 ug Primary: Anti-SAHH (orb6916) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SAHH Polyclonal Antibody, Unconjugated (orb6916) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.