SAE1 phospho S185 Antibody

SKU: orb345661

Description

SAE1 (phospho-S185) antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 2 µg/mL
• Reactivity: Human
• Application Notes:
  This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to phosphorylated SAE1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific antibody reacts with human SAE1 pS185 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein.
• Purity: This purified antibody is directed against human SUMO Activating Enzyme E1 protein. The product was purified from monospecific antiserum by affinity chromatography. This antibody is specific for human SAE1 protein phosphorylated at S185. A BLAST analysis using the sequence of the immunizing peptide was used to suggest that this antibody would react with SUMO Activating Enzyme E1 protein from human (100%), bovine, dog, chimpanzee (96%), mouse (93%), and rat (92%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.02 mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-SAE1 pS185 antibody, rabbit anti-SUMO activating enzyme subunit 1 pS185 antibody, Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, SUA1, SAE-1

Western blot using Biorbyt's Rabbit anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.

Quick Database Links

UniProt

Q9UBE0

RefSeq (Protein):NP_005491.1