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    SAE1 antibody

    Catalog Number: orb214956

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb214956
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to SAE1
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Primate, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human SAE1. The exact sequence is proprietary.
    Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetSAE1
    Entrez308384, 10055, 56459
    UniProt IDQ6AXQ0, Q9R1T2, Q9UBE0
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti AOS1 antibody, anti SUA1 antibody, anti UBLE1
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    SAE1 antibody

    Western blot analysis of SAE1 expression in HEK293T (A), K562 (B), U2OS (C), mouse testis (D), mouse muscle (E), rat testis (F), rat muscle (G) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 38 kD)

    SAE1 antibody

    Immunohistochemical analysis of SAE1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    SAE1 antibody

    Immunofluorescent analysis of SAE1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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      ELISA,  IF,  WB

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      Polyclonal

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      ELISA,  IF,  IHC-P,  WB

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      ELISA,  IHC,  WB

      Human, Mouse, Rat

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      Polyclonal

      Unconjugated

      50 μl, 100 μl
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