SUMO1 activating enzyme subunit 1 Antibody

• Catalog Number: orb556873
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SAE1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SAE1. The exact sequence is proprietary.
• Concentration: 0.72 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:100-1:500
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 38
• Target: SAE1
• UniProt ID: Q9UBE0
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 20% Glycerol
• Alternative names: SUMO1 activating enzyme subunit 1 , AOS1 , HSPC140
• Note: For research use only
• Application notes: Positive control: Mouse brain , Rat2 , A431 , H1299
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using SAE1 antibody

Immunohistochemical staining of A549 xenograft using SAE1 antibody

Immunoprecipitation analysis of HepG2 whole cell using SAE1 antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2) using SAE1 antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2) using SAE1 antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2) using SAE1 antibody