SAE1 Antibody

• Catalog Number: orb1525752
• Category: Antibodies
• Description: SAE1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 296 and 346
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SAE1
• UniProt ID: Q9UBE0
• NCBI: NP_005491.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: SUMO-1 activating enzyme E1 N subunit; UBLE1A; ubi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SAE1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SAE1 antibody orb1525752 used for IP at 3 µg per reaction.

Detection of human SAE1 by immunohistochemistry.Sample: FFPE section of human bladder carcinoma. Antibody: Affinity purified rabbit anti-SAE1 (Cat. No. orb1525752) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB