S100B Antibody

• Catalog Number: orb1735083
• Category: Antibodies
• Description: S100B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide /S100 calcium binding protein B
• Dilution range: Western Blot 1:500 – 1:1000 Immunofluorescence 1:100 – 1:400 Immunohistochemistry (Paraffin) 1:100 Immunohistochemistry (Frozen) 1:100 – 1:200
• Conjugation: Unconjugated
• Target: S100B
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1735083 overnight at 4°C. Lysates/proteins at 35 μg per lane. Anti-S100B antibody at 1/700 dilution.

Immunohistochemical analysis of paraffin embedded Human brain tissue labeling S100B antibody with orb1735083 at 1/100.

Immunohistochemical of frozen sections. Sample: rat brain. Green: S100B (orb1735083): 1-200; Anti-rabbit 488: 1-500.