S100B Antibody

• Catalog Number: orb1252143
• Category: Antibodies
• Description: S100B Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PS1B1-1
• Tested applications: FC, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a, kappa
• Immunogen: Recombinant full-length human S100B protein was used as the immunogen for the S100B antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistology (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the S100B antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S100B
• UniProt ID: P04271
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Protein S100-B, S-100 protein beta chain, S-100 pr
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistology (FFPE): 0.5-1 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the S100B antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using S100B antibody (clone PS1B1-1). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human melanoma with S100B antibody (clone PS1B1-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human schwanoma with S100B antibody (clone PS1B1-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Confocal Immunofluorescent analysis of A2058 cells using Alexa Fluor 488-labeled S100B antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).