S100B Antibody

• Catalog Number: orb1274274
• Category: Antibodies
• Description: S100B Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: S100B/1706R
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: IgG, kappa
• Immunogen: Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The optimal dilution of the recombinant S100B antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S100B
• UniProt ID: P04271
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Protein S100-B, S-100 protein beta chain, S-100 pr
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The optimal dilution of the recombinant S100B antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human melanoma with recombinant S100B antibody (clone S100B/1706R). HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.