S100B Antibody

• Catalog Number: orb1252188
• Category: Antibodies
• Description: S100B Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4C4.9
• Tested applications: FC, IF, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: IgG2a, kappa
• Immunogen: Purified bovine brain S100 protein was used as the immunogen for this S100B antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the S100B antibody to be titered up or down for optimal performance.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S100B
• UniProt ID: P04271
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Protein S100-B, S-100 protein beta chain, S-100 pr
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the S100B antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human samples using S100B antibody (clone 4C4.9). Predicted molecular weight: 12-15 kDa.

IHC testing of human melanoma stained with S100B antibody + AEC chromogen (clone 4C4.9). No special pretreatment is required for IHC staining of formalin-paraffin tissues.

Immunofluorescence testing of A2058 cells and Alexa Fluor 488 labeled S100B antibody (clone 4C4.9).