S100A5 Antibody

• Catalog Number: orb1824420
• Category: Antibodies
• Description: S-100A5 (S100 calcium binding protein A5) is a 92 amino acid protein that belongs to the S-100 family. Containing two EF-hand domains, S-100A5 binds calcium, zinc and copper. One subunit can simultaneously bind two calcium ions or two copper ions plus one zinc ion. Calcium and copper ions compete for the same binding sites on the S-100A5 protein.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S100A5/7472
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 1-92) from the human protein was used as the immunogen for the S100A5 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This S100A5 antibody is available for research use only.
• UniProt ID: P33763
• Storage: Aliquot the S100A5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human brain tissue with S100A5 antibody (clone S100A5/7472). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free S100A5 antibody (clone S100A5/7472) as confirmation of integrity and purity.