S100A4 Antibody

• Catalog Number: orb1252181
• Category: Antibodies
• Description: S100A4 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S100A4/1482
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG1, kappa
• Immunogen: Human recombinant S100A4 protein was used as the immunogen for this S100A4 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTThe optimal dilution of the S100A4 antibody for each application should be determined by the researcher.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S100A4
• UniProt ID: P26447
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: S100A4, S100 calcium binding protein A4, 18A2, 42A
• Note: For research use only
• Application notes: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RTThe optimal dilution of the S100A4 antibody for each application should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

IHC testing of FFPE human placenta with S100A4 antibody (clone S100A4/1482). Required HIER: steam sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling.

SDS-PAGE Analysis of Purified, BSA-Free S100A4 Antibody (clone S100A4/1482). Confirmation of Integrity and Purity of the Antibody.