S-tag Rabbit Polyclonal Antibody

• Catalog Number: orb500331
• Category: Antibodies
• Description: S-tag Rabbit Polyclonal Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: All
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide S-tag
• Tested applications: ELISA, WB
• Dilution range: WB=1:1000-5000, ELISA=1:1000-5000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Stag; S tag; Ribonuclease A; KETAAAKFERQHMDS.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: S-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Lane 2: S-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Primary: Anti-S-tag (orb500331) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD.