RYK antibody

• Catalog Number: orb1473912
• Category: Antibodies
• Description: Mouse monoclonal antibody to RYK
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RYK. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RYK
• Entrez: 6259
• UniProt ID: P34925
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: JTK5A; Tyrosine-protein kinase RYK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RYK expression in human lung (A) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 68 kD)

Flow cytometric analysis of A431 cells using Anti-RYK Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.