RUNX2 Rabbit Polyclonal Antibody

• Catalog Number: orb10256
• Category: Antibodies
• Description: RUNX2 Rabbit Polyclonal Antibody
• Target: RUNX2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human RUNX2 (202-300/521aa)
• UniProt ID: Q13950
• RRID: AB_10746538
• MW: 57(hu)/67(mo,rat) kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RUNX2_HUMAN; Runx-2; Runt-related Transcription Fa
• Note: For research use only

References

Zhang, Ping et al. Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones Cell Tissue Res, 350, 425-437 (2012)

PubMed: 22955562

Applications

IF

Zhang, Ci et al. Bone regeneration in minipigs by intrafibrillarly-mineralized collagen loaded with autologous periodontal ligament stem cells Sci Rep, 7, 10519 (2017)

PubMed: 28874877

Applications

IHC

Reactivity

Porcine

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-RUNX2 antibody (orb10256), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Mouse thyroid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RUNX2) Polyclonal Antibody, Unconjugated (orb10256) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Large intestine (Mouse) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-RUNX2 (orb10256) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-65 kD, Observed band size: 60 kD.

Sample: Lane 1: Mouse Colon tissue lysates, Lane 2: Mouse Lymph node tissue lysates, Lane 3: Rat Colon tissue lysates, Lane 4: Rat Lymph node tissue lysates, Primary: Anti-RUNX2 (orb10256) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57/67 kDa, Observed band size: 60 kDa.