Runx2 Rabbit Polyclonal Antibody

SKU: orb573718

Description

Rabbit polyclonal antibody to Runx2

Research Area

Epigenetics & Chromatin, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: WB
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide corresponding to a region of Rat
• Target: Runx2
• Molecular Weight: 57 kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

Cbfa1, OSF-2

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Mouse and rat have 66 kDa, 65 kDa, 57 kDa and 51 kDa isoforms that contain this peptide sequence.

25 ug of the indicated Mouse and Rat tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Recommended dilution for this antibody is 1-3 ug/ml. Two isoforms are present at ~70 kDa and ~54 kDa, peptide is present in several predicted isoforms for both Mouse and Rat.

WB Suggested Anti-Runx2 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Rat Liver.

Quick Database Links

Gene Symbol

Runx2

UniProt

Q9Z2J9

RefSeq (Protein):NP_445922