RTN4 Antibody

• Catalog Number: orb1239667
• Category: Antibodies
• Description: RTN4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: NogoA antibody was raised against a 19 amino acid synthetic peptide from near the center of human NogoA.The immunogen is located within amino acids 200 - 250 of NogoA.
• Concentration: 1 mg/mL
• Dilution range: NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 131 kDa Observed: ~180 kDa
• Target: RTN4
• UniProt ID: Q9NQC3
• NCBI: NP_065393
• Storage: NogoA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: NogoA Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NogoA Antibody: ASY, NSP, NOGO, NOGOC, RTN-X, NOGO
• Note: For research use only
• Application notes: NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NogoA in human brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 µg/mL.

Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 µg/mL.

Immunofluorescence of NogoA in Mouse Brain tissue with NogoA antibody at 20 µg/mL.

Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 5 µg/ml.

Immunohistochemistry of NogoA in mouse brain tissue with NogoA Antibody at 5 µg/mL.

Immunofluorescence of NogoA in mouse brain tissue with NogoA Antibody at 20 µg/mL. Green: NogoA antibody (orb1239667) Red: Phylloidin staining Blue: DAPI staining.