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Catalog Number | orb1239349 |
---|---|
Category | Antibodies |
Description | RTN4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | NogoA antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human NogoA.The immunogen is located within the first 50 amino acids of NogoA. |
Concentration | 1 mg/mL |
Dilution range | NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, NogoA typically migrates at 180 kDa in an SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | RTN4 |
UniProt ID | Q9NQC3 |
NCBI | NP_065393 |
Storage | NogoA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | NogoA Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | NogoA Antibody: ASY, NSP, NOGO, NOGOC, RTN-X, NOGO Read more... |
Note | For research use only |
Application notes | NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, NogoA typically migrates at 180 kDa in an SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of NogoA in mouse brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 µg/mL.
Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 µg/mL.
Immunofluorescence of NogoA in Mouse Brain cells with NogoA antibody at 20 µg/mL.
ELISA, ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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