Respiratory Syncytial Virus

SKU: orb1570994

Description

Respiratory Syncytial Virus

Research Area

Infectious Disease & Virology; Respiratory Research

Key Properties

• Purification: Virus was grown on monolayer of MA cells. When the cultures showed pronounced c.p.e. virus containing medium was purified from cell fragments by low-speed centrifugation (4000 rpm, 40 min). Virus was pelleted by high-speed centrifugation at 18000 rpm for 2.5 hours in the SW27 rotor. The virus pellet was collected, diluted with STE buffer and layered over linear 30 to 60 % sucrose gradient prepared in STE buffer. The sample was recentrifuged at 25000 rpm in SW27 rotor for 2.5 h at + 4 °C, and then the virus band was collected, diluted with STE buffer and pelleted by centrifugation at 30000 rpm for 1.5 h at + 4 °C. The pellet of virus was collected and diluted in STE buffer.

Storage & Handling

• Storage: -20 °C (-15 … -30 °C allowed)
• Buffer/Preservatives: STE, 0.09 % sodium azide (NaN₃), 0.005 % thimerosal
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only