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Respiratory Syncytial Virus
SKU: orb1570994
Description
Images & Validation
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Key Properties
−| Purification | Virus was grown on monolayer of MA cells. When the cultures showed pronounced c.p.e. virus containing medium was purified from cell fragments by low-speed centrifugation (4000 rpm, 40 min). Virus was pelleted by high-speed centrifugation at 18000 rpm for 2.5 hours in the SW27 rotor. The virus pellet was collected, diluted with STE buffer and layered over linear 30 to 60 % sucrose gradient prepared in STE buffer. The sample was recentrifuged at 25000 rpm in SW27 rotor for 2.5 h at + 4 °C, and then the virus band was collected, diluted with STE buffer and pelleted by centrifugation at 30000 rpm for 1.5 h at + 4 °C. The pellet of virus was collected and diluted in STE buffer. |
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Storage & Handling
−| Storage | -20 °C (-15 … -30 °C allowed) |
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| Buffer/Preservatives | STE, 0.09 % sodium azide (NaN₃), 0.005 % thimerosal |
| Expiration Date | 6 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
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Respiratory Syncytial Virus (orb1570994)
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