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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20μg/ml (100μl/well) can bind human LIMPII with a linear ranger of 31. 25 - 1000 ng/mL. |
| Target | RSPO1 |
| Tag | His Tag |
| MW | 28.7 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Ser 21 - Ala 263 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human RSPO1 Protein [orb3002497]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 27.8 KDa. Observed: 40 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgR-spondin-1 Recombinant Protein [orb1228968]
Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
26.6 kD
Human Cells
0.05 mgMouse RSPO1 Protein [orb428226]
Greater than 90.0% as determined by SDS-PAGE.
Sf9, Baculovirus cells
2 μg, 10 μg, 100 μgHuman RSPO1 Protein [orb611178]
Greater than 95.0% as determined by SDS-PAGE.
CHO cells
5 μg, 20 μg, 0.1 mg

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Human R-Spondin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001033722.1 |
|---|
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Protocol Information
Rspondin 1 Recombinant Protein (orb1227894)
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