RSL1D1 Rabbit Polyclonal Antibody

• Catalog Number: orb704476
• Category: Antibodies
• Description: RSL1D1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse RSL1D1 (311-410/452aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: RSL1D1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CATX 11; CATX-11; CATX11; Cellular senescence inhi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wang, Wenjing et al. Karyopherin ? deficiency contributes to human preimplantation embryo arrest J Clin Invest, 133, e159951 (2023)

PubMed: 36647821

Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RSL1D1) Polyclonal Antibody, Unconjugated (orb704476) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Heart (Rat) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Muscle (Rat) Lysate at 40 ug, Lane 4: Muscle (Mouse) Lysate at 40 ug, Lane 5: Placenta (Rat) Lysate at 40 ug, Lane 6: Placenta (Mouse) Lysate at 40 ug, Lane 7: Cerebrum (Rat) Lysate at 40 ug, Lane 8: Cerebrum (Mouse) Lysate at 40 ug, Lane 9: Testis (Rat) Lysate at 40 ug, Lane 10: Testis (Mouse) Lysate at 40 ug, Lane 11: Uterus (Rat) Lysate at 40 ug, Lane 12: Uterus (Mouse) Lysate at 40 ug, Primary: Anti-RSL1D1 (orb704476) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 52 kD.