RPS6KB2 Mouse Monoclonal Antibody

• Catalog Number: orb783404
• Category: Antibodies
• Description: RPS6KB2 Mouse Monoclonal Antibody
• Target: RPS6KB2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human RPS6KB2 (His-tagged).
• UniProt ID: Q9UBS0
• MW: 53 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: G8F7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 70 kDa ribosomal protein S6 kinase 2; p70 beta; p7
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human hepatocarcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RPS6KB2) Monoclonal Antibody, Unconjugated (orb783404) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Jurkat cell lysates, Primary: Anti-RPS6KB2 (orb783404) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 68 kD.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Primary: Anti-RPS6KB2 (orb783404) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 68 kD.