RPLP1 antibody

• Catalog Number: orb382015
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RPLP1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: Recombinant full length protein of human RPLP1
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RPLP1
• Entrez: 6176, 100360522, 140661
• UniProt ID: P05386, P19944
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: RRP1; 60S acidic ribosomal protein P1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RPLP1 expression in SW480 (A), mouse spleen (B), mouse brain (C), rat lung (D), rat liver (E) whole cell lysates. (Predicted band size: 8; 11 kD; Observed band size: 17 kD)

Immunofluorescent analysis of RPLP1 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.