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Catalog Number | orb1530479 |
---|---|
Category | Antibodies |
Description | RPA32, Phospho (S4/S8) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | surrounding serine 4/8 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:500 - 1:5,000 |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | RPA32, Phospho (S4/S8) |
UniProt ID | P15927 |
NCBI | NP_002937.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | replication factor A protein 2; RPA32; RP-A p34; R Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Phospho RPA32 (S4/S8) by western blot.Samples: Whole cell lysate from BeWo and AlphaTC1 Clone 9 cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody at 0.1 µg/ml.
Detection of human Phospho RPA32 (S4/S8) by IHC. Sample: FFPE section of human breast carcinoma, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1, 000.
Detection of mouse Phospho RPA32 (S4/S8) by immunohistochemistry. Sample: FFPE section of mouse gut, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1, 000.
Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells treated with or without etoposide. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody.
ICC, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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