RPA32, Phospho (S4/S8) Antibody

• Catalog Number: orb1530478
• Category: Antibodies
• Description: RPA32, Phospho (S4/S8) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: surrounding serine 4/8
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:500 - 1:5,000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RPA32, Phospho (S4/S8)
• UniProt ID: P15927
• NCBI: NP_002937.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: replication factor A protein 2; RPA32; RP-A p34; R
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho RPA32 (S4/S8) by western blot.Samples: Whole cell lysate from BeWo and AlphaTC1 Clone 9 cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody at 0.1 µg/ml.

Detection of human Phospho RPA32 (S4/S8) by IHC. Sample: FFPE section of human breast carcinoma, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1, 000.

Detection of mouse Phospho RPA32 (S4/S8) by IHC. Sample: FFPE section of mouse gut, MOCK treatment (left) and CIP treatment (right). Antibody: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) used at a dilution of 1:1, 000.

Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells treated with or without etoposide. Antibodies: Affinity purified rabbit anti-Phospho RPA32 (S4/S8) antibody.