RPA1 antibody

• Catalog Number: orb1474312
• Category: Antibodies
• Description: Mouse monoclonal antibody to RPA1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: Purified recombinant human RPA70 protein fragments expressed in E.coli.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: RPA1
• Entrez: 68275, 6117
• UniProt ID: P27694, Q8VEE4
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: REPA1; RPA70; Replication protein A 70 kDa DNA-bin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RPA1 expression in Hela (A), A431 (B), MCF7 (C), COS7 (D), HT1080 (E), K562 (F) whole cell lysates. (Predicted band size: 68 kD; Observed band size: 68 kD)

Immunofluorescent analysis of RPA1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.