ROCK2 Rabbit Polyclonal Antibody

• Catalog Number: orb585033
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ROCK2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Porcine, Rabbit, Rat, Zebrafish
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 153kDa
• Target: ROCK2
• UniProt ID: O75116
• Protein Sequence: Synthetic peptide located within the following region: KNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQL
• NCBI: NP_004841
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: ROCK-II
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry with Small intestine tissue at an antibody concentration of 5 ug/ml using anti-ROCK2 antibody (orb585033).

Rabbit Anti-ROCK2 Antibody, Catalog Number: orb585033, Formalin Fixed Paraffin Embedded Tissue: Human Urinary Bladder Tissue, Observed Staining: Plasma membrane, Nucleus, Primary Antibody Concentration: 1:600, Other Working Concentrations: N/A, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.

ROCK2 antibody - middle region (orb585033) validated by WB using OVCAR-3 cell lysate at 1.0 ug/ml.

WB Suggested Anti-ROCK2 Antibody, Positive Control: Lane 1: 80 ug mouse brain extract, Lane 2: 80 ug rat brain extract, Primary Antibody Dilution: 1:500, Secondary Antibody: IRDye 800 CW goat anti-rabbit, Secondry Antibody dilution: 1:20000.