ROCK1 antibody

• Catalog Number: orb556838
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ROCK1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ROCK1. The exact sequence is proprietary.
• Concentration: 0.78 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 , IP: 1:100-1:500
• Form/Appearance: Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 158
• Target: ROCK1
• UniProt ID: Q13464
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.025% ProClin 300. 1XPBS pH 7, 1% rAlbumin, 20% Glycerol
• Alternative names: Rho associated coiled-coil containing protein kina
• Note: For research use only
• Application notes: Positive control: rat brain , NIH-3T3 , C2Cl2 , HeLa
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using ROCK1 antibody

Immunohistochemical staining of human breast carcinoma using ROCK1 antibody

Immunoprecipitation analysis of 293T whole cell using ROCK1 antibody

Western blot analysis of Various whole cell using ROCK1 antibody

Western blot analysis of NIH-3T3 whole cell(Lane 1), C2Cl2 whole cell(Lane 2) using ROCK1 antibody

Western blot analysis of rat brain lysate using ROCK1 antibody

Western blot analysis of Various whole cell using Thymidylate synthase antibody