ROCK1 Antibody

• Catalog Number: orb1261296
• Category: Antibodies
• Description: ROCK1 Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: [H5E4]
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human ROCK1
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 158kDa
• Target: ROCK1
• UniProt ID: Q13464
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated p
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ROCK1 antibody (orb1261296). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse thyroid using ROCK1 antibody (orb1261296) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human thyroid using ROCK1 antibody (orb1261296) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using ROCK1 antibody (orb1261296) at dilution of 1:100 (40x lens).