ROC1 Antibody

• Catalog Number: orb1524790
• Category: Antibodies
• Description: ROC1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ROC1
• UniProt ID: P62877
• NCBI: NP_055063.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: RING finger protein 75; ZYP protein; ROC1; RNF75;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse ROC1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (T; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-ROC1 antibody orb1524790 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human ROC1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ROC1 (Cat. No. orb1524790) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human ROC1 by immunohistochemistry.Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti-ROC1 (Cat. No. orb1524790) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB