RNLS Antibody

• Catalog Number: orb1247032
• Category: Antibodies
• Description: RNLS Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-VSIDNKKRNI
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38+35kDa bands observed in Human Heart lysates, 37kDa band observed in Mouse Heart lysates and approx 35kDa in Rat Heart lysates (calculated MW of 37.8 and 34.9 according to Human NP_001026879.2 and NP_060833.1 resp, of 33.3kDa according to Mouse NP_001161290.1 and of 35.0kDa according to Rat NP_001014189.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RNLS
• UniProt ID: Q5VYX0
• NCBI: NP_001026879.2, NP_060833.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RNLS, renalase, C10orf59, chromosome 10 open readi
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38+35kDa bands observed in Human Heart lysates, 37kDa band observed in Mouse Heart lysates and approx 35kDa in Rat Heart lysates (calculated MW of 37.8 and 34.9 according to Human NP_001026879.2 and NP_060833.1 resp, of 33.3kDa according to Mouse NP_001161290.1 and of 35.0kDa according to Rat NP_001014189.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
• Expiration Date: 12 months from date of receipt.

orb1247032 (1 ug/ml) staining of Human Heart (A), Mouse Heart (B), Rat Heart (C) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247032 (2 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.