RNF74 Rabbit Polyclonal Antibody

• Catalog Number: orb2455
• Category: Antibodies
• Description: RNF74 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Human, Porcine, Rat, Sheep
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RAG1/RNF74 (351-450/1043aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 115 kDa
• Target: RAG1
• UniProt ID: P15918
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: RAG-1; E3 ubiquitin-protein ligase RAG1; RAG-1; RA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Mouse Spleen Cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-RNF74 antibody (orb2455), dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-RNF74 (orb2455) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 130 kD.