RNF43 Rabbit Polyclonal Antibody

• Catalog Number: orb101533
• Category: Antibodies
• Description: RNF43 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RNF43 (41-140/783aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 83 kDa
• Target: RNF43
• UniProt ID: Q68DV7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: RNF124; Ring finger protein 43; URCC; RNF43_HUMAN;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Human MCF-7 cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human A549 cell lysates, Lane 4: Human HL-60 cell lysates, Primary: Anti-RNF43 (orb101533) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 83 kDa, Observed band size: 110 kDa.

Tissue/cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-RNF43 Polyclonal Antibody, Unconjugated (orb101533) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.