TRIM69 Antibody

SKU: orb1247005

Description

TRIM69 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Mouse Testis lysates (calculated MW of 57.3kDa according to Mouse NP_536771.2). Recommended concentration: 0.5-2µg/mL.Immunohistochemistry:In paraffin embedded Human Testis shows speckled staining in nuclei of spermatocytes and spermitids. Recommended concentration, 2.5-3.8µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GENKEPLHILHPQ
• Target: TRIM69
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TRIM69 , tripartite motif-containing 69, HSD34, RNF36, Trif, ring finger protein 36, trimless, tripartite motif protein 69, hCG_39321, RNF36

orb1247005 (0.5 ug/ml) staining of Mouse Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247005 (3.8 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

TRIM69

UniProt

Q86WT6

RefSeq (Protein):NP_542783.2, NP_892030.3