RNF31 Antibody

• Catalog Number: orb1245566
• Category: Antibodies
• Description: RNF31 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF31.
• Concentration: batch dependent
• Dilution range: RNF31 antibody can be used for detection of RNF31 by ELISA at 1:312500. RNF31 antibody can be used for detection of RNF31 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 120 kDa
• Target: RNF31
• UniProt ID: Q96EP0
• NCBI: NP_060469
• Storage: For short periods of storage (days) store at 4°C. For longer periods of storage, store RNF31 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: RNF31, FLJ10111, FLJ23501, MGC19975, ZIBRA, HOIP
• Note: For research use only
• Application notes: RNF31 antibody can be used for detection of RNF31 by ELISA at 1:312500. RNF31 antibody can be used for detection of RNF31 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Expiration Date: 12 months from date of receipt.

Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

Antibody used in WB on Human A549 at 1:800 (Lane 1: 50 ug hormoxia A549 lysate, Lane 2: 50 ug hypoxia A549 lysate, Lane 3: 50 ug hormoxia A549 lysate (+scrambled siRNA), Lane 4: 50 ug hypoxia A549 lysate (+scrambled siRNA), Lane 5: 50 ug hormoxia A549 lysate (RNF31 siRNA), Lane 6: 50 ug hypoxia A549 lysate (RNF31 siRNA)).