RNF25 antibody

• Catalog Number: orb345567
• Category: Antibodies
• Description: RNF25 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
• Concentration: 1.1 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:40,000, IP: 1 mg, WB: 1:200 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human RNF25 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. The antibody also recognizes mouse RNF25 protein. Reactivity against homologues from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q96BH1
• NCBI: 34878787
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Rnf25 antibody, E3 ubiquitin protein l
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 54 kDa in size corresponding to RNF25 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation analysis of human RNF25 (lane 1) or vector only control (lane 2) using RNF25 antibody

Western blot analysis of HEK293 cells using RNF25 antibody