RNF25 Antibody

• Catalog Number: orb1524077
• Category: Antibodies
• Description: RNF25 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 409 and 459
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RNF25
• UniProt ID: Q96BH1
• NCBI: NP_071898.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ring finger protein AO7; RING-type E3 ubiquitin tr
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human RNF25 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (T; 50 µg) , and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody orb1524077 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RNF25 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RNF25 antibody orb1524077 used for IP at 6 µg/mg lysate.