RNF2 Antibody, KO Validated

• Catalog Number: orb1274299
• Category: Antibodies
• Description: RNF2 Antibody, KO Validated
• Target: RNF2
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RNF2 (NP_009143.1).
• UniProt ID: Q99496
• MW: Observed: 38kDa
• Tested applications: ChIP, IF, IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RNF2, BAP1, DING, BAP-1, HIPI3, RING2, RING1B
• Note: For research use only

Western blot analysis of extracts from normal (control) and RNF2 knockout (KO) HeLa cells, using RNF2 antibody (orb1274299) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.