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RNF2 Antibody, KO Validated
Catalog Number: orb1274299
| Catalog Number | orb1274299 |
|---|---|
| Category | Antibodies |
| Description | RNF2 Antibody, KO Validated |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ChIP, IF, IHC, IP, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RNF2 (NP_009143.1). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 38kDa |
| Target | RNF2 |
| UniProt ID | Q99496 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | RNF2, BAP1, DING, BAP-1, HIPI3, RING2, RING1B Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts from normal (control) and RNF2 knockout (KO) HeLa cells, using RNF2 antibody (orb1274299) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
