RNF156 Rabbit Polyclonal Antibody

SKU: orb1698

Description

RNF156 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RNF156 (201-300/552aa)
• Target: MGRN1
• Molecular Weight: 61 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RNF156; 2610042J20Rik; mKIAA0544; md; nc; RGD1311862; MGRN1_HUMAN; MGRN1; Mahogunin RING finger protein 1; RING finger protein 156; RING-type E3 ubiquitin transferase MGRN1; 2.3.2.27; KIAA0544; MGRN1_MOUSE; MGRN1_RAT; mahogunin ring finger 1; mahogunin, ring finger 1; mahogunin ring finger 1, E3 ubiquitin protein ligase

Paraformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RNF156) Polyclonal Antibody, Unconjugated (orb1698) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RNF156) Polyclonal Antibody, Unconjugated (orb1698) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

MGRN1

UniProt

O60291