RNF128 Rabbit Polyclonal Antibody

• Catalog Number: orb578784
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RNF128
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RNF128
• Concentration: 1.0 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: RNF128
• UniProt ID: Q8TEB7
• Protein Sequence: Synthetic peptide located within the following region: LEEHVQSTNESLQLVNHEANSVAVDVIPHVDNPTFEEDETPNQETAVREI
• NCBI: NP_919445
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: GRAIL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.

Lanes: 1: CD4 T cell lysate from WT mice, 2: CD4 T cell lysate from WT mice treated with anti-CD3/CD28, 3: CD4 T cell lysate from WT mice treated with FK506, 4: CD4 T cell lysate from WT mice treated with anti-CD3/CD28 and FK506, 5: CD4 T cell lysate from TRPV1 KO mice, 6: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28, 7: CD4 T cell lysate from TRPV1 KO mice treated with FK506, 8: CD4 T cell lysate from TRPV1 KO mice treated with anti-CD3/CD28 and FK506. Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody Dilution: 1:10000, Gene Name: RNF128.

WB Suggested Anti-RNF128 Antibody Titration: 1.25 ug/ml, Positive Control: HepG2 cell lysate. RNF128 is supported by BioGPS gene expression data to be expressed in HepG2.