RNASE4 Rabbit Polyclonal Antibody

SKU: orb100741

Description

RNASE4 Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Equine, Mouse, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RNASE4
• Target: RNASE4
• Molecular Weight: 14 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CK820_G0047321; RAB1; RNS4; C730049F20Rik; CR201_G0032936; RNAS4_PANTR; RNASE4; RNase 4; 3.1.27.-; RNAS4_BOVIN; Ribonuclease BL4; RNAS4_HUMAN; RNAS4_MOUSE; RNAS4_PONAB; RNAS4_PIG; Ribonuclease PL3; RNAS4_RAT; RL3;

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human HUVEC cell lysates, Primary: Anti-RNASE4 (orb100741) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kDa, Observed band size: 15 kDa.

Tissue/cell: rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-RNASE4/Angiogenin Polyclonal Antibody, Unconjugated (orb100741) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

RNASE4

UniProt

P34096